Creation of thermostable l-tryptophan dehydrogenase by protein engineering and its application for l-tryptophan quantification
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چکیده
منابع مشابه
Induction by L-tryptophan and an analogue, alpha-methyl-DL-tryptophan, of the enzymes catabolizing L-tryptophan in Pseudomonas.
An investigation was made of the pattern of induction of the enzymes that metabolize l-tryptophan through kynurenic acid (the quinoline pathway) in Pseudomonas fluorescens. The first four enzymes in the pathway were not induced in the same proportions or in the same time courses. This lack of coordinate induction excludes a mechanism of regulation of these enzymes at a single site as proposed i...
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The eosinophilia-myalgia syndrome (EMS) has been associated with ingestion of L-tryptophan (L-TRP) produced by a single manufacturer. Epidemiological data implicated 1,1'-ethylidenebis (L-tryptophan) (EBT) (peak 97 or peak E) as a possible etiologic agent. We showed previously that Lewis rats treated with the L-TRP implicated in EMS develop fasciitis and perimyositis similar to those seen in hu...
متن کاملRedetermination of l-tryptophan hydrobromide
The redetermined crystal structure of the title compound, C(11)H(13)N(2)O(2) (+)·Br(-), is reported. Data collection at 100 K about three crystallographic axes resulted in a crystal structure with significantly higher precision in comparison to the two-dimensional data collected at 176 K [Takigawa et al. [(1966) Bull. Chem. Soc. Jpn, 39, 2369-2378]. The carboxyl group and indole ring system are...
متن کاملModification of tryptophan transport system and its impact on production of L-tryptophan in Escherichia coli.
The production of L-tryptophan through chemical synthesis, direct fermentation, bioconversion and enzymatic conversion has been reported. However, the role of transport system for aromatic amino acids in L-tryptophan producing strains has not been fully explored. In this study, the fact was revealed that L-tryptophan production and cell growth were affected by the modification of transport syst...
متن کاملl-Tryptophan Production by Achromobacter liquidum.
Conditions for the production of tryptophanase from Achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. The enzyme could be produced in amounts as great as 0.750 U/ml (degradation) and 0.294 U/ml (synthesis) by shaking cultures at 30 degrees C in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-Tryptophan was produce...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2019
ISSN: 0003-2697
DOI: 10.1016/j.ab.2019.05.010